隨著“蛋白質(zhì)組學”這一概念的出現(xiàn)和發(fā)展，細胞分子網(wǎng)絡領域的研究取得了巨大的進展，人們對此領域的認識也日益深入。然而，蛋白質(zhì)的冗余性、動力學特點和互作作用，使相關研究面臨巨大挑戰(zhàn)。在本書中，多位專家闡述了蛋白質(zhì)組學的各種研究技術(shù)，縱覽了本領域各研究方向的難點與可能性，提供了最新的實驗方案和具體實例。作為《分子生物學方法》系列叢書的一卷，本書簡明易懂，各章均包含針對標題的導言、必備材料與試劑的清單、分步驟且易于操作的實驗室方案、疑難問題的注意事項和易犯失誤的避免。本書專業(yè)權(quán)威、易于使用，適合作為實驗室指南用書，可以激發(fā)讀者對蛋白質(zhì)組學這一復雜且重要的領域的研究興趣。
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本書專業(yè)權(quán)威、易于使用，適合作為實驗室指南用書，可以激發(fā)讀者對蛋白質(zhì)組學這一復雜且重要的領域的研究興趣。
目錄
前言 v
撰稿人 ix
第一部分 引言
1. 蛋白質(zhì)組學簡介 3
Friedrich Lottspeich
第二部分 電泳分離
2. 高分辨率二維電泳 13
Walter Weiss and Angelika Gorg
3. 非經(jīng)典二維電泳 33
JacquelineBurre，Ilka Wittig，and Hermann Schagger
4. 蛋白質(zhì)檢測與定量技術(shù)用于基于凝膠的蛋白質(zhì)組學分析 59
Walter Weiss，F(xiàn)lorian Weiland，and Angelika Gorg
第三部分 質(zhì)譜和串聯(lián)質(zhì)譜的應用
5. 基質(zhì)輔助激光解吸電離質(zhì)譜 85
Rainer Cramer
6. 毛細管電泳質(zhì)譜聯(lián)用應用于人尿液蛋白質(zhì)組學分析與生物標志物發(fā)現(xiàn) 105
PetraZurbig，Eric Schiffer，and Harald Mischak
7. 多肽納升級液相色譜指南 123
Thomas Frohlich and Georg J.Arnold
8. 多維蛋白鑒定技術(shù) 143
Katharina Lohrig and Dirk Wolters
9. 肽段為中心的蛋白質(zhì)組學技術(shù)分析血小板蛋白 155
Oliver Simon，Stefanie Wortelkamp，and Albert Sickmann
10. 高分辨率質(zhì)譜蛋白質(zhì)組分析技術(shù)鑒定小鼠小腸20S蛋白質(zhì)組分子組成 173
Reinhold Weber，Regina Preywisch，Nikolay Youhnovski，Marcus Groettrup，and Michael Przybylski
第四部分 蛋白質(zhì)組學定量技術(shù)
11. 基于液相色譜質(zhì)譜聯(lián)用的定量蛋白質(zhì)組學 189
Michael W.Linscheid，Robert Ahrends，Stefan Pieper，and Andreas Kuhn
12. 膠內(nèi)酶切同位素標記蛋白質(zhì)技術(shù)用于相對定量 207
Carla Schmidt and Henning Urlaub
13.電噴霧質(zhì)譜技術(shù)用于血漿蛋白質(zhì)組定量分析 227
Hong Wang and Sam Hanash
第五部分 質(zhì)譜數(shù)據(jù)解讀
14. 算法和數(shù)據(jù)庫 245
Lennart Martens and Rolf Apweiler
15. 鳥槍法蛋白質(zhì)鑒定和質(zhì)譜法蛋白質(zhì)定量 261
Bingwen Lu，Tao Xu，Sung Kyu Park，and John R.Yates III
第六部分 蛋白質(zhì)修飾分析
16. 腦蛋白氧化修飾蛋白質(zhì)組學鑒定 291
Rukhsana Sultana，Marz ia Perluigi，and D.Allan Butter field
17.同位素標記、磷酸化多肽親和富集技術(shù)用于液相色譜-串聯(lián)質(zhì)譜蛋白質(zhì)組分析 303
Uma Kota，Ko-yi Chien，and Michael B.Goshe
第七部分 亞細胞蛋白質(zhì)組學
18. 細胞器蛋白質(zhì)組學：天然蛋白膠內(nèi)酶切篩選減少樣本的復雜性 325
Veronika Reisinger and Lutz A.Eichacker
19. 聚合物雙水相系統(tǒng)逆流分配法分離神經(jīng)系統(tǒng)質(zhì)膜 335
Jens Schindler and Hans Gerd NothWang
20. 液相色譜串聯(lián)質(zhì)譜技術(shù)進行擬南芥蛋白質(zhì)組分析的質(zhì)膜蛋白富集和制備 341
Srijeet K.Mitra，Steven D.Clouse，and Michael B.Goshe
第八部分 蛋白質(zhì)相互作用分析
21.Strep/FLAG(SF)-串聯(lián)親和純化標簽用于哺乳動物細胞蛋白質(zhì)復合物串聯(lián)親和純化 359
Christian Johannes Gloeckner，Karsten Boldt，Annette Schumacher，and Marius Ueffing
22. 順序多肽親和純化系統(tǒng)分離和鑒定大腸桿菌蛋白質(zhì)復合物 373
Mohan Babu，Gareth Butland，Oxana Pogoutse，Joyce Li，Jack F.Greenblatt，and AndreWEmili
23. 生物信息學方法分析蛋白質(zhì)相互作用 401
Beate Kruger and Thomas Dandekar
索引 433
(宋凱 張春秀 譯)
Contents
Preface. v
Contributors. ix
PART I INTRODUCTION
1. Introduction to Proteomics 3
Friedrich Lottspeich
PART II ELECTROPHORETIC SEPARATIONS
2. High-Resolution Two-Dimensional Electrophoresis 13
Walter Weiss and Angelika Gorg
3. Non-classical 2-D Electrophoresis 33
Jacqueline Burre，Ilka Wittig，and Hermann Sch？gger
4. Protein Detection and Quantitation Technologies for Gel-Based Proteome Analysis 59
Walter Weiss，F(xiàn)lorian Weiland，and Angelika Gorg
PART III MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY APPLICATIONS
5. MALDI MS 85
Rainer Cramer
6. Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery 105
Petra Zurbig，Eric Schiffer，and Harald Mischak
7. A Newcomer’s Guide to Nano-Liquid-Chromatography of Peptides 123
Thomas Frohlich and Georg J. Arnold
8. Multidimensional Protein Identification Technology 143
Katharina Lohrig and Dirk Wolters
9. Characterization of Platelet Proteins Using Peptide Centric Proteomics 155
Oliver Simon，Stefanie Wortelkamp，and Albert Sickmann
10. Identification of the Molecular Composition of the 20S Proteasome of Mouse Intestine by High-Resolution Mass Spectrometric Proteome Analysis 173
Reinhold Weber，Regina Preywisch，Nikolay Youhnovski，Marcus Groettrup，and Michael Przybylski
PART IV QUANTITATIVE PROTEOMICS
11. Liquid Chromatography-Mass Spectrometry-Based Quantitative Proteomics. 189
Michael W. Linscheid，Robert Ahrends，Stefan Pieper，and Andreas Kuhn
12. iTRAQ-Labeling of In-Gel Digested Proteins for Relative Quantification 207
Carla Schmidt and Henning Urlaub
13. Electrospray Mass Spectrometry for Quantitative Plasma Proteome Analysis 227
Hong Wang and Sam Hanash
PART V INTERPRETATION OF MASS SPECTROMETRY DATA
14. Algorithms and Databases 245
Lennart Martens and Rolf Apweiler
15. Shotgun Protein Identification and Quantification by Mass Spectrometry 261
Bingwen Lu，Tao Xu，Sung Kyu Park，and John R. Yates III
PART VI ANALYSIS OF PROTEIN MODIFICATIONS
16. Proteomics Identification of Oxidatively Modified Proteins in Brain 291
Rukhsana Sultana，Marzia Perluigi，and D. Allan Butterfield
17. Isotope-Labeling and Affinity Enrichment of Phosphopeptides for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry 303
Uma Kota，Ko-yi Chien，and Michael B. Goshe
PART VII SUBCELLULAR PROTEOMICS
18. Organelle Proteomics：Reduction of Sample Complexity by Enzymatic In-Gel Selection of Native Proteins 325
Veronika Reisinger and Lutz A. Eichacker
19. Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems 335
Jens Schindler and Hans Gerd Nothwang
20. Enrichment and Preparation of Plasma Membrane Proteins from Arabidopsis thaliana for Global Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry 341
Srijeet K. Mitra，Steven D. Clouse，and Michael B. Goshe
PART VIII ANALYSIS OF PROTEIN INTERACTIONS
21. Tandem Affinity Purification of Protein Complexes from Mammalian Cells by the Strep/FLAG (SF)-TAP Tag 359
Christian Johannes Gloeckner，Karsten Boldt，Annette Schumacher，and Marius Ueffing
22. Sequential Peptide Affinity Purification System for the Systematic Isolation and Identification of Protein Complexes from Escherichia coli 373
Mohan Babu，Gareth Butland，Oxana Pogoutse，Joyce Li，Jack F. Greenblatt，and AndreWEmili
23. Bioinformatical Approaches to Detect and Analyze Protein Interactions. 401
Beate Kruger and Thomas Dandekar
Index 433
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